Fig 1: Activated microglia in the brain.Histological analysis of the brain. Presence of activated microglia was found (Iba1+ cells in the red circle), but there was not significant difference between R&L stressed mice, regardless of handling, and Sentinels, suggesting that if an inflammatory process occurred in BM, it did not reach the central nervous system. Left: above a x2 magnification of negative control without Iba1 antibody, in the middle a x2 magnification of positive control with Iba1 antibody, below a 4x magnification of PVN (area inside the circle, see methods for stereotaxic coordinates). Center: a representative photomicrograph for each handling group (x5 magnification). Right: a series of images with different degrees of magnification of Iba1+ cells (x20 and x40).